A Solovyeva
Saint Petersburg State University, Russia
Title: Historically introduced by McConkey to explain the slow mutation rate of highly abundant proteins, weak protein (quinary) interactions are emergent properties of living cells. The protein complexes that result from quinary interactions are transient and thus difficult to study biochemically in vitro. Cross-correlated relaxation induced polarization transfer (CRIPT) based in-cell NMR allows the characterization of protein quinary interactions with atomic resolution inside live prokaryotic and eukaryotic cells. We showed that RNAs are an important component of protein quinary interactions. Protein quinary interactions are unique to the target protein and affect physicochemical properties, protein activity, and interactions with drugs.
Biography
Biography: A Solovyeva
Abstract
Transposable elements (TEs) are widely spread in all phylogenetic groups and comprise a significant part of eukaryotic genomes. A marine trematode – Himasthla elongata possesses an alternation of sex and parthenogenetic stages. It was believed that trematode parthenitae constitutes a clonal population. But their larvae have different infectivity rates. Thereby, the polymorphism could be expected and it increases the chance for successful host invasion. We found that the S-SAP (Sequence-Specific Amplification Polymorphism) method revealed clonal variability in the H. elongata larvae genomes. The aim was to determine the main components of the variable bands and which could be the source of clonal diversity. Cloning of several bands from S-SAP patterns and their sequence analysis allowed finding the presence of CR1-like and RTEX-like non-LTR (Long Terminal Repeat) TE fragments in the conservative regions in electrophoresis pattern and non-LTR and LTR-like fragments in variable zones. Some sequences are found in transcriptome and seem to belong to active TE copies. The fragment 7.5 cloned from variable bands doesn’t have any ORFs. Dot hybridization revealed that 7.5 prevail in high and medium molecular length bands of S-SAP patterns and it is also present transcriptome. According to PCR analysis, 7.5 seems to be a part of CR1-like elements, but it forms clusters near pericentromeric and subtelomeric zones at chromosomes i.e. near satellite DNA regions. Thus, we suppose that mobile genetic elements play a key role in trematode clonal polymorphisms occurrence.