Canan Kus
Ankara University, Turkey
Title: The synthesis and cytotoxic activities of imidazole [4,5-b]pyridine-2-phenyl carboxamidine derivatives
Biography
Biography: Canan Kus
Abstract
Some heterocyclic compounds having amidino group are present in synthetic products with a range of pharmacological effects such as antithrombotic, anti-hypercholesterolemic, antihistaminic, antihypertensive, amebicidal, antihyperglycemic, anti-inflammatory, anticancer and antibacterial activities. The synthesis of 4-(3-benzyl-3H-imidazo[4,5-b]pyridin-2-yl)benzonitrile derivatives were performed from p-cyanobenzaldehyde. These compounds were transformed to the desired amidine derivatives (Z)-N,N'-bis(4-chlorobenzyl)-4-(3-benzyl-3H-imidazo[4,5-b]pyridin-2-yl)benzamidine via the Pinner reaction followed by stirring the resulting imidates ethyl 4-(3-benzyl-3H-imidazo[4,5-b]pyridin-2-yl)benzimidate in ethanolic amines. Human breast cancer cells (MCF7) and hepatocellular carcinoma cells (HepG2) were grown in a humidified incubator supplemented with 5% CO2. Fetal bovine serum (%10) and antibiotics were added in culture media. Cytotoxic concentrations (IC50 doses) of chemicals was determined by MTT (MTT [3-(4,5-dimethyl-2-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay for 24, 48 and 72-hour time periods. The inhibition of cell proliferation was determined by measuring the optical density of the chromogenic product at 540 nm with an ELISA reader.